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The optimal temperatu

 
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BerichtGeplaatst: 19-07-2018 09:34:14    Onderwerp: The optimal temperatu Reageren met citaat

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why temperature control in the IVF lab is crucial?

Temperature control in the IVF lab is crucial , as it plays an important role in the success of any IVF cycle.

The primary function of an ART laboratory is to provide an optimal environment for gametes and embryos to grow. The success of any ART cycle is very much dependant on the Quality Control (QC) and Quality Assurance (QA) maintained in the IVF laboratory. Optimization of the laboratory procedures is of great interest to the scientists in the field of IVF.

All important IVF equipment's used in IVF laboratory has direct or indirect contact with embryos, gametes or the culture media. These all should be handled and monitored in an accurate and precise way. Fine temperature regulation is critical to embryology laboratories aiming to maximize in vitro fertilization (IVF) and development http://www.newjerseydevilsteamstore.com/adidas-ryane-clowe-jersey , implantation and pregnancy of assisted reproductive technique (ART).

It is common practice to maintain a temperature of 37 degree C during all working and culture conditions in the IVF laboratory. This will allow for an optimal environment for the handling and culture of gametes and embryos.

The three most important physical conditions in the IVF laboratory that can be controlled are the temperature, pH and osmolality of the IVF culture medium. Laboratories are required to document and monitor these physical conditions regularly as part of their ongoing QCQA programs. Monitoring and documentation of temperatures inside incubators, refrigerators and freezers is an integral part of routine day to day QC in the IVF laboratory.

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The optimal temperature range for human IVF culture is assumed to be between the limits of 36.7°C and 37.0°C; deviations above and below this range should be regarded as criticaldangerous. The effects of sub optimal temperature control can be manifested as fragmentation, delayed cell division, or reduced pregnancy rates due to induced chromosomal abnormalities that jeopardize implantation or result in early pregnancy loss, particularly at the pre-clinical stage (biochemical pregnanciesblighted ova). Therefore every effort should be made to ensure that there is no temperature fluctuation during any laboratory manipulation that involves the handling of oocytes or embryos and that the temperature is maintained at 37°C.

Oocytes need to be kept at body temperature (36–37°C) as much as possible. There should .
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